Transgenic Plant resistant to virus disease base on RNA – interferance technology
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Transgenic Plant resistant to virus disease base on RNA – interferance technology
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Viet Nam
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There are over 2000 plant viruses have been detected and studied, about half of them attack and cause serious diseases in plants. The damage due to virus disease on crop is very serious can be up to 95 – 100%, the affect not only relate to decline in productivity but also to the quality of harvest products. In this work, tobacco plants were used as a model to study the possibility of application of RNAi technique in order to make transgenic plants resistant to two virus causing mosaic disease, CMV (cucumber mosaic virus) and TMV (Tobacco mosaic virus). The segments of gene encoding coat protein (CP) of TMV and CMV virus were uesd to construct transformation vector carrying ihpRNA CP genes (intron hairpin RNA) correspond to each virus (CM – CPI and TMV – CPI) or both viruses (CMV – T – CPI), respectively. The ihpRNA CP constructs were then transformed into tobacco plants (Nicotiana tobacum, cv. K326) via Agrobacterium tumefaciens. In order to evaluate the resistance capacity of transgenic plants against studied viruses, TO generation transgenic tobacco plants carrying CMV – CPI, TMV – CPI or CMV – T – CPI were inoculated with each CMV or TMV, respectively. The results obtained from analysis of transgenic plants after three times virus challenge using PCR, RT – PCR and ELISA indicated the complete resistance capacity of transgenic tobacco plants against CMV, TMV, or both virusees with the corresponding rate about 64.6%, 87% and 70.8% of examined lines.
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Transgenic Plant resistant to virus disease base on RNA – interferance technology