| Virus disease survey on tomato plants in Don Duong and Duc Trong region (Province Lam Dong, Vietnam) using Das – Elisa tests | |
| The growing area of tomato in Don Duong and Duc Trong districts (Lam Dong province, Vietnam) is approximately 4000 ha yearly yielding more than 12,000 tons of fruit. Based on the symtomatology and host range analysis, four viruses including tomato leaf curl virus (TLCV), tomato yellow leaf curl virus (TYLCV), tomato mosaic virus (TMV), and cucumber mosaic virus (CMV) were isolated from the leafs of disease plants and identified by DAS-ELISA tests. In three years (2006 – 2008), more than 1000 samples were collected and tested for virus infection. It was examined that virus disease was the main cause of severe harvest losses of tomato in this region. Mixed infection was detected on many yields, and the range and types of infected viruses changed from season to season. The aphid and transmission tests were also carried out, and the results showed that silverleaf whitefly (Bemisia tabaci) was the major vector of virus transmission. | |
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| Application of viscosity method for determination of molecular weight of Poly L(+) Lactic acid for evaluation of the Polymerization reaction | |
| Poly L(+) Lactic Acid (PLA) is product from polycondensation reaction in solvent bulk using Tin metallic powder. Determination of molecular weight of PLA by viscosity method is a simple technique and simultaneous evaluation of molecular weight in the relative large range (M= 104-106). The comparison of results from Gel Permeation Chromatography (GPC), PLA melting points and viscosity methods showed the good correlation. The difference between PLA molecular weights which measured by viscosity and GPC methods is about 11%. Results from viscosity method showed that it can be used for quick, simple and low cost measurement in comparison with GPC method for evaluation of influences of granule Sn catalyst sizes on polycondensation reaction. This method was applied for investigation of influence of catalyst size on the polycondensation of L(+) lactic acid to produce PLA | |
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| The PCR Kit to diagnostic Greening diseases | |
| Greening disease is the most destructive disease of citrus in many countries in the worl. It in very serve in Vietnam. One of the most powerful tool to diagnostic this disease is PCR. However, it is very expensive and complex, not easy for provincal centers to diagnostic. The experiment were carried out to improve the protocol with the aim to reduce the cost of testing disease and also to reduce the risk to the environment due to reducing a lot of toxic chemicals. The results are included: Use centrifuge at room temperature instead of use cool centrifugate were not effect to PCR results with the Primers OI I/OI 2 c. In the DNA extraction steps, the chemical were reduced from 750µl of Cloroform: isopropanol to 550µl isopropanol from 650µl to 450µl and TE 1X form 100µl to 70µl, the PCR result were 100%. The primer OI 1/OI 2 c concentration was reduced from 0.5µl to 0.2µl the PCR result were 100%. The clearness of the band were the same at all concntration of this primers. The concentration of Taq DNA polymerase were reduced from 1.25U to 0.6U but PCR results were 100%. The PCR Kit to diagnostic Greening diseases were gaven good results after one month produced (stored at 4oC). Concentration of Tween 20 at 0.05% and 0.03% were given good rusults (PCR is 1005) (store at 4oC). | |
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